Nanodimentional Aggregates In Organic Monolayers Studied With Atomic Force Microscopy (AFM) And Fluorescence Lifetime Imaging Microscopy (FLIM)

Details Nanodimentional Aggregates In Organic Monolayers Studied With Atomic Force Microscopy (AFM) And Fluorescence Lifetime Imaging Microscopy (FLIM) Nanodimentional Aggregates In Organic Monolayers Studied With Atomic Force Microscopy (AFM) And Fluorescence Lifetime Imaging Microscopy (FLIM)

Apr 2007

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2007aipc..899..257i&link_type=abstract

SIXTH INTERNATIONAL CONFERENCE OF THE BALKAN PHYSICAL UNION. AIP Conference Proceedings, Volume 899, pp. 257-258 (2007).

Mathematics

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Langmuir-Blodgett Films On Solids, Polymers On Surfaces, Biological Molecules On Surfaces, Time-Resolved Optical Spectroscopies And Other Ultrafast Optical Measurements In Condensed Matter

Scientific paper

Organic monolayers from a fluorescently labeled phospholipid (DPPE-NBD) were deposited on solid supports under special conditions that form stable nanometer wide bilayers cylinders that protrude from the monolayer. This molecule was frequently used in sensor applications due to its sensitivity to environment changes. The proposed configuration should provide both fast response times (ultra thin film) and increased sensitivity (greatly increased surface area). AFM can clearly distinguish between the different phases. The height difference between the solid-expanded and the liquid-expanded phase was measured to be 1.4 nm while the bilayer thickness was 5.6 nm. The solid domains show a 20 % decrease in fluorescence lifetime in comparison to the monolayer as measured by FLIM. This difference in lifetimes is explained in the model of fluorescence self quenching in the solid phase due to the molecules being closer to each other.

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