Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis

Details Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis

2008-07-08

arxiv.org/abs/0807.1276v1

Methods in molecular biology (Clifton, N.J.) 432 (2008) 83-100

Biology

Quantitative Biology

Genomics

Scientific paper

10.1007/978-1-59745-028-7_6

Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial proteins with two-dimensional electrophoresis (2DE), showing some adjustment over the classical techniques to improve resolution of the mitochondrial proteins. This covers both the protein solubilization, the electrophoretic part per se, and the protein detection on the gels, which makes the interface with the protein identification part relying on mass spectrometry.

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