Biology – Quantitative Biology – Genomics
Scientific paper
Dec 2010
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2010agufm.p13b1384a&link_type=abstract
American Geophysical Union, Fall Meeting 2010, abstract #P13B-1384
Biology
Quantitative Biology
Genomics
[0456] Biogeosciences / Life In Extreme Environments, [0465] Biogeosciences / Microbiology: Ecology, Physiology And Genomics
Scientific paper
The occurrence of microbes in the atmosphere has been the subject of scientific inquiry since Louis Pasteur’s time; however, data on the nature and diversity of microbial life in the upper troposphere and stratosphere is very limited. To experimentally address this, we have designed, constructed, and field-tested a lightweight, autonomous system that can sample at high altitudes using a latex sounding balloon. An important aspect of our sampling protocol is the ability to decontaminate and assess the level of background contamination during laboratory and field handling. Our approach involves the parallel decontamination and monitoring of 3 identical payloads: (i) one that remains in the laboratory, (ii) a control on the flight string, and (iii) a payload that opens and samples airborne particles in the atmosphere. Comparative analysis of various sterilization methods indicated that ethylene oxide was most effective at decreasing the concentration of DNA-containing cells, decreasing background cellular contamination by 94%. In conjunction, germicidal ultraviolet light, sodium hypochlorite, and 70% ethanol were used to decrease the concentration of microbes associated with payload surfaces. Bioaerosol collection is achieved by impact sampling on a 3.5 mm^2 retention surface covered with a thin layer of sterile silicone grease as the payload travels through the atmosphere. Initial flights have been successful in recovering viable microorganisms present in parcels of air at altitudes of 3 km to 9 km. Microscopic analysis on the collected cell assemblages implied that ~70% of the cells were potentially viable, and aerobic heterotrophic bacteria were cultured and isolated from liquid and agar-solidified culture media. Future plans include increasing the sampling altitude up to ~30 km in a series of discrete steps, maintaining our background controls and connection to lower altitude measurements. The pressure, temperature, and radiation levels in Earth’s stratosphere are similar to those on the surface of Mars. Thus, investigating life at altitudes of 30 km may indicate whether life could exist on extraterrestrial surfaces and provide the experimental technology and rationale to sample the atmospheres of other planets and moons in the solar system.
Adkins W. P.
Bryan N.
Christner Brent C.
Giammanco J. R.
Guzik Greg T.
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