Metabolically deuterated species determined in rat cerebella by FT-IR microspectroscopy as a novel probe of brain metabolism

Details Metabolically deuterated species determined in rat cerebella by FT-IR microspectroscopy as a novel probe of brain metabolism Metabolically deuterated species determined in rat cerebella by FT-IR microspectroscopy as a novel probe of brain metabolism

Jun 1998

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1998aipc..430..294w&link_type=abstract

The eleventh international conference on fourier transform spectroscopy. AIP Conference Proceedings, Volume 430, pp. 294-297 (1

Other

Spectra, Photodissociation, And Photoionization, Luminescence, Infrared And Raman Spectroscopy

Scientific paper

Deuterated brain tissue in the cerebella of adult rats (from ingesting 40% D2O prior to their being sacrificed) provides a means of studying brain metabolism without the use of radioisotopes. Microtomed frozen sections of the brains of adult rats were examined with FT-IR microspectroscopy. Corresponding brain sections of control animals (100% H2O) were used for comparison. Multiple branches in the cerebella of several deuterated brains and control brains were mapped across the molecular layer, granular cell layer, and white matter to the opposite granular cell and molecular layers. Individual layers of the cerebella were compared for CD and OD or ND contents. Also, the absorbance of the CD band relative to that of other forms was compared among the molecular layer, the granular cell layer, and the white matter. Speculation concerning the relative metabolic uptake of deuterium was possible by comparing the absorbance of deuterated species with that of the ordinary hydrogen isotope form of the same functional group of the same tissue specimen.

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