MassChroQ: A versatile tool for mass spectrometry quantification

Details MassChroQ: A versatile tool for mass spectrometry quantification MassChroQ: A versatile tool for mass spectrometry quantification

2011-09-26

arxiv.org/abs/1109.5488v1

Proteomics, volume 11, issue 17, pages 3572-3577, September 2011

Biology

Quantitative Biology

Quantitative Methods

23 pages, 8 figures

Scientific paper

10.1002/pmic.201100120

Recently, many software tools have been developed to perform quantification in LC-MS analyses. However, most of them are specific to either a quantification strategy (e.g. label-free or isotopic labelling) or a mass-spectrometry system (e.g. high or low resolution). In this context, we have developed MassChroQ, a versatile software that performs LC-MS data alignment and peptide quantification by peak area integration on extracted ion chromatograms. MassChroQ is suitable for quantification with or without labelling and is not limited to high resolution systems. Peptides of interest (for example all the identified peptides) can be determined automatically or manually by providing targeted m/z and retention time values. It can handle large experiments that include protein or peptide fractionation (as SDS-PAGE, 2D-LC). It is fully configurable. Every processing step is traceable, the produced data are in open standard format and its modularity allows easy integration into proteomic pipelines. The output results are ready for use in statistical analyses. Evaluation of MassChroQ on complex label-free data obtained from low and high resolution mass spectrometers showed low CVs for technical reproducibility (1.4%) and high coefficients of correlation to protein quantity (0.98). MassChroQ is freely available under the GNU General Public Licence v3.0 at http://pappso.inra.fr/bioinfo/masschroq/.

Affiliated with

Also associated with

No associations

Rating

MassChroQ: A versatile tool for mass spectrometry quantification does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with MassChroQ: A versatile tool for mass spectrometry quantification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and MassChroQ: A versatile tool for mass spectrometry quantification will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-688550