MACROscopic imaging of tumor xenografts using fluorescence, phase contrast, and transmitted light

Details MACROscopic imaging of tumor xenografts using fluorescence, phase contrast, and transmitted light MACROscopic imaging of tumor xenografts using fluorescence, phase contrast, and transmitted light

Oct 2004

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2004spie.5578..149c&link_type=abstract

Photonics North 2004: Optical Components and Devices. Edited by Armitage, John C.; Fafard, Simon; Lessard, Roger A.; Lampropoul

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Recent advances in imaging technology have contributed greatly to biological science. Confocal fluorescence microscopes (CFM) can acquire 2D and 3D images of biological samples such as live or fixed cells and tissues. Specimens that are large (e.g., a 10 mm x 10 mm tissue section) and overfill the field of view (FOV) of typical microscope objectives require use of image tiling to cover the entire specimen. This can be time consuming and cause artefacts in the composite image. The MACROscope system (Biomedical Photometrics Inc, Waterloo, Canada), is a confocal device with a 22 mm x 70 mm FOV; ideal for imaging large tissue sections in a single frame. The system used here is a prototype capable of simultaneous acquisition from up to three detection channels. Fluorescence images of SiHa mouse tumour xenografts stained with CD31-Cy3, showing blood vessel location, and EF5-Cy5, showing areas of tissue hypoxia, were collected. Differential phase contrast (DPC) images of the same section were also recorded to show tissue morphology. Finally, RGB transmitted light images of human tongue and pancreas tissues were obtained. This new device avoids the need for image tiling and provides simultaneous imaging of multiple fluorescently-labeled tissue specific markers in large biological samples. This enables time- and cost-efficient high-throughput screening of (immuno)histopathological samples. This device may also serve in the imaging of high-throughput DNA and tissue arrays.

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