Computer Science
Scientific paper
Oct 1960
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1960natur.188..318g&link_type=abstract
Nature, Volume 188, Issue 4747, pp. 318-320 (1960).
Computer Science
Scientific paper
DE DUVE et al.1 have described the occurrence in rat liver of a new type of cytoplasmic particle, the lysosome, which was characterized by the following properties: (1) A distinct enzymic composition, containing a number of hydrolytic enzymes (acid phosphatase, β-glucuronidase, acid ribonuclease and acid deoxyribonuclease). (2) They can be isolated as a distinct intermediate fraction, between the mitochondria and microsomes, by differential centrifugation. (3) When isolated undamaged, the lysosomes show a low level of enzyme activity, but when the membrane is ruptured by freezing, and thawing, or by the addition of non-ionic detergents such as `Triton-X-100', there is a considerable increase in the activity of the enzymes.
Greenbaum A. L.
Slater Timothy F.
Wang Ding Yi
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