Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses

Details Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses

2006-10-30

arxiv.org/abs/q-bio/0610056v1

Proc Natl Acad Sci U S A (20/10/2006) sous presse

Biology

Quantitative Biology

Neurons and Cognition

Scientific paper

10.1073/pnas.0603511103

Synaptic vesicle (SV) exocytosis mediating neurotransmitter release occurs spontaneously at low intraterminal calcium concentrations and is stimulated by a rise in intracellular calcium. Exocytosis is compensated for by the reformation of vesicles at plasma membrane and endosomes. Although the adaptor complex AP-3 was proposed to be involved in the formation of SVs from endosomes, whether its function has an indirect effect on exocytosis remains unknown. Using mocha mice, which are deficient in functional AP-3, we identify an AP-3-dependent tetanus neurotoxin-resistant asynchronous release that can be evoked at hippocampal mossy fiber (MF) synapses. Presynaptic targeting of the tetanus neurotoxin-resistant vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) is lost in mocha hippocampal MF terminals, whereas the localization of synaptobrevin 2 is unaffected. In addition, quantal release in mocha cultures is more frequent and more sensitive to sucrose. We conclude that lack of AP-3 results in more constitutive secretion and loss of an asynchronous evoked release component, suggesting an important function of AP-3 in regulating SV exocytosis at MF terminals.

Affiliated with

Also associated with

No associations

Rating

Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-231301