Laboratory Studying of Metabolic Activity of Terrestrial Bacteria in Subsurface Martian Environments

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Abstract Large amount of water ice presents in subsurface layers of Mars according to observational data. This ice is able to intensive sublimation through porous upper regolith if the surface is heated enough by sunlight. TES data give the value of maximal daytime temperature for some areas of martian surface up to 300 K. Under such conditions vapours diffuse through the porous surface layer. As a result the "wet layer" appears under sand's surface. In our experiment we used special vacuum chamber to model the process of ice sublimation and vapour diffusion while heating. This chamber has a cryogenic pump inside for effective capturing of water vapour. The atmospheric pressure inside was maintained at 0.1-0.01 mbar. We use water ice sample covered by several centimetres of sand with weight fraction of organic matter (glucose) ~ 10-5-10-4. Sublimation is driven by radiation heating of sand's surface. Temperature control of sand's surface is also provided. We have discovered the increase of bacterial population of Vibrio Sp. in the "wet layer". These results confirm that shallow ground ice and a few hours of heating per day could provide sufficient conditions for growth of bacterial population under martian surface. Environments of some areas of modern Mars are close to that we have modelled in our experiments. We also present upgraded experimental setup especially constructed for studying of metabolism of different types of bacteria and different chemical composition of soil. Further upgrade is in progress at present and it will allow us to vary atmospheric composition inside the chamber and measure products of metabolism such as methane etc.

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