Isotopic inhomogeneity of leaf water: Evidence and implications for the use of isotopic signals transduced by plants

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Variations as large as 11%. in 18 O values and 50%. in D values were observed among different fractions of water in leaves of ivy ( Hedera helix ) and sunflower ( Helianthus annuus ). This observation contradicts previous experimental approaches to leaf water as an isotopically uniform pool. Using ion analysis of the water fractions to identify sources within the leaf, we conclude that the isotopic composition of the water within cells, which is involved in biosynthesis and therefore recorded in the plant organic matter, differs substantially from that of total leaf water. This conclusion must be taken into account in studies in which isotope ratios of fossil plant cellulose are interpreted in paleoclimatic terms. In addition, our results have implications for attempts to explain the Dole effect and to account for the variations of 18 O / 16 O ratios in atmospheric carbon dioxide, since the isotopic composition of cell water, not of total leaf water, influences the 18 O values of O 2 and CO 2 released from plants into the atmosphere.

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