Infrared spectroscopic imaging microscopy: Applications to biological systems

Mathematics – Logic

Scientific paper

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Infrared And Raman Spectroscopy, X-Ray Imaging

Scientific paper

The coupling of imaging modalities with spectroscopic techniques adds additional dimensions to sample analysis in both the spectroscopic and spatial domains. The particular ability of infrared (IR) imaging to explore the spatial distribution of chemically distinct species on length scales ranging from microns to kilometers demonstrates the versatility and diversity of spectroscopic imaging. In this paper, we focus on the further development of our Fourier-transform (FT) based mid-IR spectroscopic imaging technique which combines the analytical capabilities of mid-IR spectroscopy with the morphological information obtained from optical imaging. The seamless combination of spectroscopy for molecular analysis with the power of visualization represents the future of infrared microscopy. Our spectroscopic imaging instrument integrates several infrared focal-plane arrays with a Michelson step-scan interferometer, generating high-fidelity and high spectral resolution mid-infrared spectroscopic images. The instrumentation produces multidimensional, chemically specific images, while simultaneously obtaining high resolution spectra for each detector pixel. The spatial resolution of the images approaches the diffraction limit for mid-infrared wavelengths, while the spectral resolution is determined by the interferometer, and can be 4 cm-1 or higher. Data derived from a variety of materials, particularly biological samples, illustrate the capabilities of the technique for readily visualizing chemical complexity and for providing statistical data on sample heterogeneity.

No associations

LandOfFree

Say what you really think

Search LandOfFree.com for scientists and scientific papers. Rate them and share your experience with other people.

Rating

Infrared spectroscopic imaging microscopy: Applications to biological systems does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Infrared spectroscopic imaging microscopy: Applications to biological systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Infrared spectroscopic imaging microscopy: Applications to biological systems will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFWR-SCP-O-1330413

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.