Biology – Quantitative Biology – Subcellular Processes
Scientific paper
2005-11-12
J Theor Biol 240: 464-474 (2006)
Biology
Quantitative Biology
Subcellular Processes
In press, Journal of Theoretical Biology. 27 pages, 9 figures
Scientific paper
10.1016/j.jtbi.2005.10.007
Integrating biological information from different sources to understand cellular processes is an important problem in systems biology. We use data from mRNA expression arrays and chemical kinetics to formulate a metabolic model relevant to K562 erythroleukemia cells. MAP kinase pathway activation alters the expression of metabolic enzymes in K562 cells. Our array data show changes in expression of lactate dehydrogenase (LDH) isoforms after treatment with phorbol 12-myristate 13-acetate (PMA), which activates MAP kinase signaling. We model the change in lactate production which occurs when the MAP kinase pathway is activated, using a non-equilibrium, chemical-kinetic model of homolactic fermentation. In particular, we examine the role of LDH isoforms, which catalyze the conversion of pyruvate to lactate. Changes in the isoform ratio are not the primary determinant of the production of lactate. Rather, the total concentration of LDH controls the lactate concentration.
Ahn Natalie G.
Betterton M. D.
Downer Joshua
Resing Katheryn A.
Sevinsky Joel R.
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