Hollow cathode ion lasers for deep ultraviolet Raman spectroscopy and fluorescence imaging

Details Hollow cathode ion lasers for deep ultraviolet Raman spectroscopy and fluorescence imaging Hollow cathode ion lasers for deep ultraviolet Raman spectroscopy and fluorescence imaging

Dec 2001

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2001rsci...72.4452s&link_type=abstract

Review of Scientific Instruments, Volume 72, Issue 12, pp. 4452-4459 (2001).

Computer Science

18

Visible And Ultraviolet Spectrometers, Gas Lasers Including Excimer And Metal-Vapor Lasers, Analytical Methods Involving Vibrational Spectroscopy, Visible And Ultraviolet Sources, Infrared And Raman Spectroscopy, Laser Spectroscopy

Scientific paper

This article describes the development of hollow cathode ion lasers and their use in constructing an ultraviolet micro-Raman spectrograph with native fluorescence imaging capability. Excitation at 224.3 nm is provided by a helium-silver hollow cathode metal ion laser and at 248.6 nm by a neon-copper hollow cathode metal ion laser. Refractive microscope objectives focus chopped continuous wave laser light on a sample and collect 180° scattered photons. Imaging is accomplished by broadband visible illumination and by deep ultraviolet laser induced excitation of visible wavelength native fluorescence in untagged micro-organisms. This makes possible a detection strategy employing rapid imaging with laser excitation to locate regions of native fluorescence activity, followed by deep ultraviolet resonance Raman spectroscopy of the identified fluorescent sites. We have employed this probe for in situ detection of micro-organisms on mineral and soil substrates. We present here the deep ultraviolet resonance Raman spectra for the gram negative iron reducing bacterium Shewanella oneidensis obtained while the micro-organism remains in situ on the unpolished surface of the mineral calcite and in a Mars soil analog, JSC1. In the current configuration the in situ mineral surface limit of detection for fluorescence is one organism in 2×104 μm2 field of view and of order 20-30 micro-organisms for Raman spectra. For the Mars soil sample analog fluorescent target selection gives an effective ultraviolet resonance Raman spectral detection limit of 6×104cells/gm or ~60 ppb.

Affiliated with

Also associated with

No associations

Rating

Hollow cathode ion lasers for deep ultraviolet Raman spectroscopy and fluorescence imaging does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Hollow cathode ion lasers for deep ultraviolet Raman spectroscopy and fluorescence imaging, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hollow cathode ion lasers for deep ultraviolet Raman spectroscopy and fluorescence imaging will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-1374673