Biology
Scientific paper
Dec 1999
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1999spie.3755..163m&link_type=abstract
Proc. SPIE Vol. 3755, p. 163-173, Instruments, Methods, and Missions for Astrobiology II, Richard B. Hoover; Ed.
Biology
Scientific paper
A moss plant dating as much as 40,000 years old was collected from the permafrost of the Kolyma Lowlands of Northeastern Siberia. The plant tissue was revived and cultured for the extraction of its genomic DNA. Using the polymerase chain reaction technique, the 18S ribosomal RNA gene was cloned and its sequence studied. Comparative sequence analysis of the cloned ribosomal DNA to other known 18S RNA showed very high sequence identity and was revealed to be closest to the moss specie, Aulacomnium turgidum. The results of this study also show the ability of biological organisms to rest dormant in deep frozen environments where they can be revived and cultured under favorable conditions. This is significant in the notion that celestial icy bodies can be media to preserve biological function and genetic material during long term storage or transport.
Gilichinsky David A.
Hoover Richard B.
Marsic Damien
Ng Joseph D.
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