Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions

Details Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions

Oct 2003

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2003adspr..32.1561g&link_type=abstract

Advances in Space Research, Volume 32, Issue 8, p. 1561-1567.

Computer Science

Scientific paper

We compared quantitatively vinculin-related adhesion parameters in osteoblastic cells submitted to opposite mechanical stresses, i.e., low deformation and frequency strain regimens (stretch condition) and microgravity exposure (relaxed condition). Cyclic deformation induced a biphasic response comprising new focal contacts formation followed by their clustering in ROS cells. Microgravity exposure induced a reduction in focal contact number and clustering in ROS cells. We previously demonstrated that 1% cyclic deformations at 0.05 Hz during a daily 10 min episode over 7 days stimulated ROS 17/2.8 growth as compared to static culture whereas relaxed ROS proliferated similarly to static culture (BC). To evaluate whether the proliferation (stretch) or the survival (relaxed) status of ROS cells influences focal contact organization, we inhibited ERKs proliferative-dependent pathway. Inhibition of proliferation by PD98059 was overcome although not fully restored by stretch. Furthermore stretch-induced clustering of vinculin-positive contacts still occurs in the presence of ERKs inhibitor, whereas the increase in focal contact number is abolished. In conclusion, we showed that focal contacts are mechanoeffectors and that hyper-mechanical stimulation could up regulate focal contacts size as compared to hypo-mechanical that down regulate clusterization.

Affiliated with

Also associated with

No associations

Rating

Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-912315