Excitation wavelength tunable Raman spectroscopy: study of preresonance enhancement of hemoglobin with 700-860 nm excitation wavelengths

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We have developed an excitation wavelength tunable Raman system which consists of a spectrometer, a CCD detector and an electronically tuned Ti:sapphire (ETT) laser. All those components are controlled with a single computer. This system is useful for Raman excitation profile measurements. A new technique was developed to calibrate deviation of wavelengths of ETT laser from that of Raman spectrometer within +/- 1.5cm-1 of error. Obtained Raman spectra of hemoglobin measured with various excitation wavelengths in 700-860 nm range showed characteristic change of their intensities in some of Raman bands. Assignments of Raman bands were carried out sufficiently in help with change in intensity of the bands which reflect symmetric character of the responsible vibrational modes with the change in excitation wavelength.

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