Biology – Quantitative Biology – Genomics
Scientific paper
2006-11-24
Proteomics 3 (03/2003) 249-53
Biology
Quantitative Biology
Genomics
website publisher http://www.interscience.wiley.com
Scientific paper
10.1002/pmic.200390037
The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two-dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commercially available nonionic detergents, dodecyl maltoside and decaethylene glycol mono hexadecyl ether proved most efficient. They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics.
Luche Sylvie
Rabilloud Thierry
Santoni Véronique
No associations
LandOfFree
Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-540442