Essential absorption data for In-vitro and In-vivo near infrared spectrometric biotic fluid assays

Computer Science – Performance

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Infrared And Raman Spectroscopy, Infrared Spectrometers, Auxiliary Equipment, And Techniques

Scientific paper

The performance of near infrared spectroscopic assays for the analysis of biotic samples within the frame of clinical chemistry has been the subject of several investigations. The determination of metabolites such as glucose can be carried out using multivariate calibration exploiting spectral data from different intervals. The reliability of such assays can be improved if special boundary conditions are fulfilled, as the existence of other components with similar spectra can lead to severe systematic errors for concentration prediction. Absorptivity data between 11000 and 3500 cm-1 is presented for the monosaccharides glucose, fructose and galactose, as well as for ethanol and hydroxyethylstarch used for hemodilution or as blood plasma expander. Pure compounds and aqueous solutions were studied. Since the hydrogen bonding network is considerably influenced by such solutes, also spectral information is provided on the variability of the spectra of aqueous samples due to temperature and electrolyte content of NaCl and KCl.

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