Mathematics – Logic
Scientific paper
Aug 1994
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994adspr..14..289v&link_type=abstract
Advances in Space Research, Volume 14, Issue 8, p. 289-298.
Mathematics
Logic
1
Scientific paper
To study the effects of weightlessness on mouse fetal long bone rudiment growth and mineralization we have developed a tissue culture system for the Biorack facility of Spacelab. The technique uses standard liquid tissue culture medium, supplemented with Na-β-glycerophosphate, confined in gas permeable polyethylene bags mounted inside ESA Biorack Type I experiment containers. The containers can be flushed with an air/5% CO2 gas mixture necessary for the physiological bicarbonate buffer used. Small amounts of fluid can be introduced at the beginning (e.g. radioactive labels for incorporation studies) or at the end of the experiment (fixatives). A certain form of mechanical stimulation (continuous compression) can be used to counteract the, possibly, adverse effect of μ-gravity. Using 16 day old metatarsals the in vitro calcification process under μ-gravity conditions can be studied for a 4 day period.
Brouwer T.
Maas Philipp
van Loon Jack J. W. A.
van Vilsteren M.
Veldhuijzen Paul J.
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