Statistics – Methodology
Scientific paper
Jan 1994
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994gecoa..58..365c&link_type=abstract
Geochimica et Cosmochimica Acta, vol. 58, Issue 1, pp.365-375
Statistics
Methodology
Scientific paper
The hydrogen isotopic measurement ( D) of the non-exchangeable hydrogens in herbivore bone collagen has potential for paleoclimate research. We have developed the methodology for extracting the hydrogen from collagen for isotopic analysis and for correcting the D results for hydrogen exchange. Preparations of whole bone powders, demineralized bone, or gelatin extracts from fresh bone samples all give reliable D results and have isotopic results, yields, and proportions of exchangeable hydrogens consistent with that expected for collagen. Gelatin extraction for removal of contaminants remains a valuable option for the study of fossil bone samples. Vacuum preheating under good vacuum at 150°C for two days for whole bone powders and at 100°C for one day for gelatins is an important step to remove all adsorbed water before samples are oxidized for isotopic analysis. Of the remaining hydrogens released following oxidation, 20.5% in whole bone powders and 23.1% in gelatin extracts exchange with laboratory atmospheric water vapor within 48 hours. The D results can be corrected for this exchange and for minor effects of sample preparation by using a calibration bone standard to determine the D value of laboratory water vapor.
Cormie Allison B.
Gray James
Schwarcz Henry P.
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