Biology – Quantitative Biology – Quantitative Methods
Scientific paper
2006-10-17
Biology
Quantitative Biology
Quantitative Methods
33 pages 6 figures 3 tables
Scientific paper
We have developed a luminescence-measurement system for liquid bio/chemiluminescence that can obtain quantitative luminescence spectra as the absolute total number of luminescence photons at each wavelength or photon energy and quantum yields. Calibration of light-collection efficiency in the system is performed with a reference double-plate cell. This method is applicable to sample cells of any kind suitable for measurement, which is a great advantage over previous techniques in practical experiments. Using this system, the quantum yield of aqueous luminol chemiluminescence was obtained as 1.23-+0.20%, which is in good agreement with previously reported values.
Akiyama Hidefumi
Ando Yoriko
Enomoto Toshiteru
Irie Tsutomu
Kubota Hidehiro
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