Mathematics – Logic
Scientific paper
Jan 2004
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2004adspr..34.1373k&link_type=abstract
Advances in Space Research, Volume 34, Issue 6, p. 1373-1377.
Mathematics
Logic
Radiation Risk, Detection Of Microlesions, Heavy Ions, Fluorescent Dye, Cell Irradiation
Scientific paper
In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.
Koniarek Jan P.
Thomas Jean-Louis
Vazquez Manuel
No associations
LandOfFree
Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1838521