Cloning Human Fetal γ Globin and Mouse α -Type Globin DNA: Characterization and Partial Sequencing

Details Cloning Human Fetal γ Globin and Mouse α -Type Globin DNA: Characterization and Partial Sequencing Cloning Human Fetal γ Globin and Mouse α -Type Globin DNA: Characterization and Partial Sequencing

Dec 1978

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1978sci...202.1284s&link_type=abstract

Science, Volume 202, Issue 4374, pp. 1284-1289

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6

Scientific paper

Two globin-related clones isolated from collections of bacteriophages containing unfractionated Eco RI fragments of human and mouse DNA were characterized. Charon3AHs51.1Hbγ includes 2.7 kilobase pairs of human DNA containing a large part of a fetal γ globin chain structural gene; Charon 3AMm30.5 includes 4.7 kilobase pairs of mouse DNA related to α globin. The human fetal γ globin gene has within its coding region two intervening sequences of noncoding DNA, IVS 1 and IVS 2, of approximately 100 and 900 base pairs. Sequence IVS 1 is located at the position of one of the two intervening sequences occurring in adult globin genes; IVS 2 is located at the position of the other.

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