Computer Science
Scientific paper
Jun 1998
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1998aipc..430..286l&link_type=abstract
The eleventh international conference on fourier transform spectroscopy. AIP Conference Proceedings, Volume 430, pp. 286-289 (1
Computer Science
Infrared And Raman Spectroscopy, Infrared Spectrometers, Auxiliary Equipment, And Techniques
Scientific paper
Fourier transform infrared microspectroscopy can be used to collect infrared spectra from microscopic regions of tissue sections. If spectra are collected along a grid pattern, then maps of chemical functional groups can be produced and correlated to tissue histopathology. In the present study, white matter from multiple sclerosis and control brains were examined. Mapping experiments were designed such that 17 spectra were collected at 200 μm intervals along a line that was partially or wholly within a multiple sclerosis lesion site or within a representative white matter region of control tissue. Data analysis was based on earlier in vitro studies, which found that the carbonyl at 1740 cm-1 increases when lipids become oxidized, and the amide I peak at ~1660 cm-1 broadens when proteins become oxidized. The results indicated that the C=O to CH2 ratio (1740 cm-1:1468 cm-1) was elevated at several collection points in lesion sites from each of five multiple sclerosis brains examined compared to values from white matter of four control brains. Inspection of the amide I peak at 1657 cm-1 revealed that it was broadened towards 1652 cm-1 in multiple sclerosis tissues but not control tissues. These results suggest that lipids and proteins are oxidized at active multiple sclerosis lesion sites. The localization of these products to lesion sites supports a role for free radicals in the pathogenesis of multiple sclerosis.
Levine Steven M.
Wetzel David L.
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