Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA Cloning

Details Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA Cloning Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA Cloning

Apr 1977

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1977sci...196..161b&link_type=abstract

Science, Volume 196, Issue 4286, pp. 161-169

Mathematics

Logic

35

Scientific paper

The Charon λ bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF. We present here some of the data on which the safety of these systems was evaluated. DNA fragments ranging in size from 0 to 2.2 × 104 base pairs can be cloned in these EK2 Charon phages.

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