Biochemical Characterization of HydF, a Scaffolding Enzyme, in the Synthesis of the Hydrogenase Active Site Metal Center: Implications Towards the Evolution of Biocatalysts from Mineral-based Components on Early Earth

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Scientific paper

[FeFe]-hydrogenase active site biosynthesis utilizes radical chemistry
on a scaffold protein whose ancestor may have been one of the earliest
examples of a protein that couples the chemistry of an Fe-S peptide nest
with a nucleotide binding nest.

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