Alkylating agent (MNU)-induced mutation in space environment

Details Alkylating agent (MNU)-induced mutation in space environment Alkylating agent (MNU)-induced mutation in space environment

Jan 2001

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2001adspr..28..563o&link_type=abstract

Advances in Space Research, Volume 28, Issue 4, p. 563-568.

Mathematics

Logic

Scientific paper

In recent years, some contradictory data about the effects of microgravity on radiation-induced biological responses in space experiments have been reported. We prepared a damaged template DNA produced with an alkylating agent (N-methyl-N-nitroso urea; MNU) to measure incorrect base-incorporation during DNA replication in microgravity. We examined whether mutation frequency is affected by microgravity during DNA replication for a DNA template damaged by an alkylating agent. Using an in vitro enzymatic reaction system, DNA synthesis by Taq polymerase or polymerase III was done during a US space shuttle mission (Discovery, STS-91). After the flight, DNA replication and mutation frequencies were measured. We found that there was almost no effect of microgravity on DNA replication and mutation frequency. It is suggested that microgravity might not affect at the stage of substrate incorporation in induced-mutation frequency.

Affiliated with

Also associated with

No associations

Rating

Alkylating agent (MNU)-induced mutation in space environment does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Alkylating agent (MNU)-induced mutation in space environment, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alkylating agent (MNU)-induced mutation in space environment will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-921662