Advances in photon counting for bioluminescence

Statistics – Applications

Scientific paper

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Scientific paper

Photon counting systems were originally developed for astronomy, initially by the astronomical community. However, a major application area is in the study of luminescent probes in living plants, fishes and cell cultures. For these applications, it has been necessary to develop camera system capability at very low light levels -- a few photons occasionally -- and also at reasonably high light levels to enable the systems to be focused and to collect quality images of the object under study. The paper presents new data on MTF at extremely low photon flux and conventional ICCD illumination, counting efficiency and dark noise as a function of temperature.

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