Abundance and stable carbon isotope composition of amino acids in molecular weight fractions of fossil and artificially aged mollusk shells

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Amino acid abundances in molecular weight fractions (separated by ultrafiltration) of artificially aged carbonate shells and fossil shells are reported. Hydrolyzable amino acids were primarily concentrated in the <500 Dalton fraction and in an acid insoluble fraction. With continued heating, proteins hydrolyzed to free amino acids and peptides. The amount of amino acids in the acid insoluble fraction diminished whereas the amount of amino acids in the <500 Dalton molecular weight fraction increased. The amount of soluble peptides in molecular weight fractions between 500 and 10,000 Daltons was minimal in both the heated and fossil shells. The extent of amino acid racemization was not uniform throughout the molecular weight spectrum. Free amino acids and amino acids in lower molecular weight peptides were racemized to the greatest extent, whereas amino acids recovered from the insoluble residue fractions were the least racemized. The 13 C values of individual amino acids in the molecular weight fractions of the artificially aged shells generally varied by 2-3 relative to their respective values in the total hydrolyzate of the unheated shell. These differences may in part reflect fractionations resulting from hydrolysis and decomposition.

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