Biology – Quantitative Biology – Genomics
Scientific paper
2006-11-24
Proteomics 4 (03/2004) 551-61
Biology
Quantitative Biology
Genomics
web publisher http://www.interscience.wiley.com
Scientific paper
10.1002/pmic.200300589
The influence of thiol blocking on the resolution of basic proteins by two-dimensional electrophoresis was investigated. Cysteine blocking greatly increased resolution and decreased streaking, especially in the basic region of the gels. Two strategies for cysteine blocking were found to be efficient: classical alkylation with maleimide derivatives and mixed disulfide exchange with an excess of a low molecular weight disulfide. The effect on resolution was significant enough to allow correct resolution of basic proteins with in-gel rehydration on wide gradients (e.g. 3-10 and 4-12), but anodic cup-loading was still required for basic gradients (e.g. 6-12 or 8-12). These results demonstrate that thiol-related problems are not solely responsible for streaking of basic proteins on two-dimensional gels.
Chevallet Mireille
Diemer Hélène
Dorsselaer Alain van
Leize-Wagner Emmanuelle
Luche Sylvie
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