Biology – Quantitative Biology – Genomics
Scientific paper
2006-11-27
Methods in molecular biology (Clifton, N.J.) 355 (2006) 111-20
Biology
Quantitative Biology
Genomics
website publisher http://www.interscience.wiley.com
Scientific paper
A new, versatile, multiphasic buffer system for high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins in the relative molecular weight range of 300 000-3000 Da is described. The system, based on the theory of multiphasic zone electrophoresis, allows complete stacking and destacking of proteins in the above M(r) range. The buffer system uses taurine and chloride as trailing and leading ion, respectively, and Tris, at a pH close to its pK(a), as the buffering counterion. Coupled with limited variation in the acrylamide concentration, this electrophoresis system allows to tailor the resolution in the 6-200 kDa M(r) range, with minimal difficulties in the post electrophoretic identification processes.
Diemer Hélène
Dorsselaer Alain van
Lescuyer Pierre
Luche Sylvie
Rabilloud Thierry
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