A reexamination of cellulose carbon-bound hydrogen D measurements and some factors affecting plant-water D/H relationships

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Scientific paper

The method of Epstein et al . (1976) for analysis of D/H ratios of cellulose carbon-bound hydrogen has been modified. This modified "renitration" method yields D values which are in agreement with those obtained by the sodium chlorite delignification method. Comparison of results obtained by the renitration method with the published results of Epstein et al . (1976) indicate some differences in the D values of individual samples. However, the overall plant-water D relationship determined by Epstein et al ., is not greatly changed upon redetermination by the renitration method. Additional data from a variety of plants representing a wide geographical range reveal that relative humidity is an important variable in determining the D value of cellulose C-H hydrogen on this inter-regional scale. The role of relative humidity can be reasonably explained by a leaf water model that assumes an isotopic steady-state during transpiration. These results reaffirm the conclusion of Epstein et al ., that the D variations of the source water are the dominant control of the D variations of cellulose C-H hydrogen from naturally grown plants. Thus, there is an expectation that these cellulose D variations can have climate significance.

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