A branching process model for flow cytometry and budding index measurements in cell synchrony experiments

Details A branching process model for flow cytometry and budding index measurements in cell synchrony experiments A branching process model for flow cytometry and budding index measurements in cell synchrony experiments

2010-09-28

arxiv.org/abs/1009.5745v1

Annals of Applied Statistics 2009, Vol. 3, No. 4, 1521-1541

Statistics

Applications

Published in at http://dx.doi.org/10.1214/09-AOAS264 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Ins

Scientific paper

10.1214/09-AOAS264

We present a flexible branching process model for cell population dynamics in synchrony/time-series experiments used to study important cellular processes. Its formulation is constructive, based on an accounting of the unique cohorts in the population as they arise and evolve over time, allowing it to be written in closed form. The model can attribute effects to subsets of the population, providing flexibility not available using the models historically applied to these populations. It provides a tool for in silico synchronization of the population and can be used to deconvolve population-level experimental measurements, such as temporal expression profiles. It also allows for the direct comparison of assay measurements made from multiple experiments. The model can be fit either to budding index or DNA content measurements, or both, and is easily adaptable to new forms of data. The ability to use DNA content data makes the model applicable to almost any organism. We describe the model and illustrate its utility and flexibility in a study of cell cycle progression in the yeast Saccharomyces cerevisiae.

Affiliated with

Also associated with

No associations

Rating

A branching process model for flow cytometry and budding index measurements in cell synchrony experiments does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with A branching process model for flow cytometry and budding index measurements in cell synchrony experiments, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A branching process model for flow cytometry and budding index measurements in cell synchrony experiments will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-694991