3D quantitative imaging of the microvasculature with the Texas Instruments Digital Micromirror Device

Details 3D quantitative imaging of the microvasculature with the Texas Instruments Digital Micromirror Device 3D quantitative imaging of the microvasculature with the Texas Instruments Digital Micromirror Device

Nov 2001

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2001spie.4457..137f&link_type=abstract

Proc. SPIE Vol. 4457, p. 137-144, Spatial Light Modulators: Technology and Applications, Uzi Efron; Ed.

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There is a growing need for developing 3D quantitative imaging tools that can operate at high speed enabling real-time visualization for the field of biology, material science, and the semiconductor industry. We will present our 3D quantitative imaging system based on a confocal microscope built with a Texas Instruments Digital Micromirror Device (DMD). By using the DMD as a spatial light modulator, confocal transverse surface (x, y) scanning can be performed in parallel at speeds faster than video rate without physical movement of the sample. The DMD allows us to programmably configure the source and the detection pinhole array in the lateral direction to achieve the best signal and to reduce the crosstalk noise. Investigations of the microcirculation were performed on 40 g to 45 g golden Syrian hamsters fit with dorsal skin fold window chambers. FITC-Dextran or Red blood cells from donor hamsters, stained with Celltracker CM-DiI, were injected into the circulation and imaged with the confocal microscope. We will present the measured results for the axial resolution, in vivo, as well as experimental results from imaging the window chamber.

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