Isolation of Macronuclei from the Ciliate Tetrahymena pyriformis GL

Details Isolation of Macronuclei from the Ciliate Tetrahymena pyriformis GL Isolation of Macronuclei from the Ciliate Tetrahymena pyriformis GL

Dec 1965

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1965natur.208.1350l&link_type=abstract

Nature, Volume 208, Issue 5017, pp. 1350-1351 (1965).

Computer Science

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Scientific paper

SEVERAL different non-ionic detergents have recently been used for the isolation of nuclei from mammalian cells1-5. One of these detergents, `Triton X-100', was found to solubilize readily cytoplasmic components, without affecting the structural integrity (at the lightmicroscope level) of nuclei from rat liver cells and mouse plasma cell tumours5. `Triton'-isolated nuclei contained more than 90 per cent of the DNA in the original cell homogenate, retained rapidly labelled RNA and NAD-pyrophosphorylase activity5. The detergent digitonin has been used with some success for the isolation of macronuclei from stationary phase Tetrahymenapyriformis, mating type II, var. 1 (ref. 6).

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