Formation and Characterization of Marigranules from Tryptophan and Sugars

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We found that molecular oxygen and aromatic amino acids such as tryptophan, tyrosine and phenylalanine were essential for the formation of marigranules. Among aromatic amino acids, tryptophan gave the best yield of marigranules. Among indole derivatives, kynurenine gave the best yield of marigranules. Large marigranules (0.3 3 μm in diameter) were formed from tryptophan in the presence of Ca2+ and Mg2+, and small marigranules (0.2 0.6 μm in diameter) were produced in the absence of such divalent metal ions. Marigranules formed from tryptophan were partially solubilized with methanol and completely solubilized with dimethyl sulfoxide and dimethyl-formamide. The solubilized marigranules consisted of polymers with molecular weights of 2×103 and 105 107 daltons. The methanol-soluble fraction provided well-defined vesicles upon sonication. Marigranule-like particles were formed from D,L-glyceraldehyde, D-erythrose and D-ribose but they were not formed from glycolaldehyde, L-arabinose and D-glucose. Among sugars, D-erythrose gave the best yield of the particles.

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