Photo-Chemical Activation of Adenine

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Scientific paper

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Scientific paper

EARLIER1 it was found that adenine sulphate on irradiation by a mercury arc acquired growth-promoting properties. The material was tested on rats, receiving a synthetic basal diet deficient in vitamin B, supplemented by ox kidney extract as a source of vitamin B2. Since those preliminary experiments a large number of tests have been carried out with rats and pigeons. In most experiments adenine sulphate in aqueous suspension (slightly acidified) was exposed to a Hanovia Home Sun (220 volts, consuming 2.1 amp.) at 15 cm. distance for ½-l hour. The irradiated product has proved unable to cure `polyneuritis' in pigeons even in 5 mgm. doses. But the same daily dose supported growth in 36 young rats which had ceased to grow on a diet composed of starch, caseinogen and McCollum's salt mixture supplemented by cod liver oil and an aqueous extract of ox kidney, which provided vitamin B2 and a small quantity of vitamin B1. Irradiated adenine gave no response in young rats (31 in number), receiving ox liver extract or autoclaved marmite as the source of vitamin B2. Irradiated guanine chloride, cytosine chloride and uracil did not resemble the action of irradiated adenine in preliminary experiments (7 rats were used). Irradiated adenine could not replace vitamin B2 in the diet.

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