Mathematics – Logic
Scientific paper
Aug 1994
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994adspr..14...87l&link_type=abstract
Advances in Space Research, Volume 14, Issue 8, p. 87-96.
Mathematics
Logic
Scientific paper
For the study of gravity's role in the processes of plant cell differentiation in vitro, a model ``seed-seedling-callus'' has been used. Experiments were carried out on board the orbital stations Salyut-7 and Mir as well as on clinostat. They lasted from 18 to 72 days. It was determined that the exclusion of a one-sided action of gravity vector by means of clinostat and spaceflight conditions does not impede the formation and growth of callus tissue; however, at cell and subcellular levels structural and functional changes do take place. No significant changes were observed either on clinostat or in space concerning the accumulation of fresh biomass, while the percentage of dry material in space is lower than in control. Both in microgravity (MG) and in control, even after 72 days of growth, cells with a normally developed ultrastructure are present. In space, however, callus tissue more often contains cells in which the cross-section area of a cells, a nuclei and of mitochondria are smaller and the vacuole area - bigger than in controls. In microgravity a considerable decrease in the number of starch-containing cells and a reduction in the mean area of starch grains in amyloplasts is observed. In space the amount of soluble proteins in callus tissue is 1.5 times greater than in control. However, no differences were observed in fractions when separated by the SDS-PAGE method. In microgravity the changes in cell wall material components was noted. In the space-formed callus changes in the concentration of ions K, Na, Mg, Ca and P were observed. However, the direction of these changes depends on the age of callus. Discussed are the possible reasons for modification of morphological and metabolic parameters of callus cells when grown under changed gravity conditions.
Kenstavičienė P.
Laurinavičius R.
Necitailo G.
Rupainiene O.
No associations
LandOfFree
In Vitro plant cell growth in microgravity and on clinostat does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with In Vitro plant cell growth in microgravity and on clinostat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In Vitro plant cell growth in microgravity and on clinostat will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1493359