Computer Science
Scientific paper
Feb 2004
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2004spie.5251..154g&link_type=abstract
Detectors and Associated Signal Processing. Edited by Chatard, Jean-Pierre; Dennis, Peter N. J. Proceedings of the SPIE, Volume
Computer Science
Scientific paper
We present a fluorescence detection system for capillary analysis. It is designed using a CMOS BDJ (Buried Double p-n Junction) detector which can be operated either as a photodiode or as a wavelength-sensitive device. Noise-reduction techniques such as signal pre-amplification and synchronous detection are implemented to boost the sensitivity of measurements. The system indicates fluorescence intensity for concentration determination, and average wavelength of fluorescence spectrum for molecular discrimination. The system has been tested by measuring two widely used fluorophores (FITC and Rhodamine B) in different concentrations. A 407-nm blue laser diode and a 532-nm green YAG compact laser have been respectively employed for their excitation. The illuminated volume inside the capillary is about 5 nl. The best results have been obtained with FITC, enabling as low as 10-10 M to be detectable.
Blum Loïc
Galvan Jean-Marc
Leca-Bouvier Béatrice
Lu Guo-Neng
Pittet Patrick
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