Sources of neutral lipids in a temperate intertidal sediment

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Scientific paper

The compositions of sterol, alcohol and ketone fractions from an oxic intertidal surface sediment from Corner Inlet. Victoria, Australia, have been examined by capillary gas chromatographymass spectrometry and related to the lipids of diatoms cultured from the sediment and to lipids of the sea-grass Zostera muelleri . Of the more than thirty sterols in the sediment most appear to derive from diatoms, including the major sterol 24-methylcholesta-5.22E-dien-3 -ol. Small amounts of 24-ethylcholesterol probably derive from Zostera , with a minor diatom contribution. 5 -stanols, both fully saturated and with a side-chain double bond, represented ca . 14% of the total sterols: a significant proportion of these are probably derived from marine invertebrates. These organisms also contribute C 26 sterols. most of the cholesterol and possibly small amounts of -sterols. The data suggest that in situ biohydrogenation of stenols was not a major process in sterol transformation in the surface oxic sediment. Alcohols ranged from C 12 to C 30 and showed an unusual abundance of unsaturated components with 9 -16:1 being the major constituent. The long-chain alcohols probably originate largely from Zostera but the shorter-chain. C 12 -C 20 . alcohols are at least partly derived from wax esters of unknown origin. Diatoms do not contribute significant amounts of alcohols. Ketones were not major constituents of the sediment and consisted mainly of 6.10.14-trimethylpentadecan-2-one and a series of n -alkan-2-ones ranging from C 16 to C 33 . The latter showed a similar distribution to that of the n -alkanes but the correspondence was not sufficient to substantiate a product-precursor relationship. Very long-chain C 3 - C 39 unsaturated methyl and ethyl ketones, which may originate from the marine unicellular alga Emiliania huxleyi . were present in low concentration.

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