Kinetics of the hydrolysis of guanosine 5'-phospho-2-methylimidazolide

Details Kinetics of the hydrolysis of guanosine 5'-phospho-2-methylimidazolide Kinetics of the hydrolysis of guanosine 5'-phospho-2-methylimidazolide

Mar 1986

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1986orli...17...85k&link_type=abstract

Origins of Life and Evolution of the Biosphere, Volume 17, Issue 1, pp.85-103

Other

2

Scientific paper

We have studied the hydrolysis of guanosine 5'-phospho-2-methylimidazolide, 2-MeImpG, in aqueous buffered solutions of various pH's at 75°C and 37°C. At 75°C and pH≤1.0, two kinetic processes were observed spectrophotometrically: the first and more rapid one is attributed to the hydrolysis of the phosphoimidazolide P-N bond; the second and much slower one, to the cleavage of the glycosidic bond. At 37°C, pH 2.0, the spectrophotometrically determined rate constant of P N bond hydrolysis was confirmed by using high pressure liquid chromatography, HPLC. With the latter technique it was possible to separate reactants and products and also to extend the pH-rate profile into the neutral region where rates are slower and, therefore, difficult to measure spectrophotometrically. The pH-rate profiles at both temperatures exhibit similar behavior. At pH<2 the pseudo-first-order rate constant increases with decreasing pH; in the region 27. These data are consistent with a reactivity order zwitterion>anion for P N bond hydrolysis. It is noteworthy that P N bond hydrolysis in phosphoimidazolides is very slow compared to other phosphoramidates. This may be one of the reasons why this compound showed extraordinary ability in forming long oligomers under template-directed conditions.

Affiliated with

Also associated with

No associations

Rating

Kinetics of the hydrolysis of guanosine 5'-phospho-2-methylimidazolide does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Kinetics of the hydrolysis of guanosine 5'-phospho-2-methylimidazolide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kinetics of the hydrolysis of guanosine 5'-phospho-2-methylimidazolide will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-1286176